artificial skin – culturing of different skin cell lines for generating an artificial skin substitute on cross-weaved spider silk fibres人工皮肤u2014u2014培养不同的细胞系生成人造皮肤替代cross-weaved蜘蛛丝纤维.pdfVIP

Artificial Skin – Culturing of Different Skin Cell Lines for Generating an Artificial Skin Substitute on Cross-Weaved Spider Silk Fibres 1 . 1. 1 1 ¨ 1 Hanna Wendt * , Anja Hillmer , Kerstin Reimers , Joern W. Kuhbier , Franziska Schafer-Nolte , 1 2 1 Christina Allmeling , Cornelia Kasper , Peter M. Vogt 1 Department of Plastic, Hand, and Reconstructive Surgery, Medical School Hannover, Hannover, Germany, 2 Institute of Technical Chemistry, Leibniz University of Hannover, Hannover, Germany Abstract Background: In the field of Plastic Reconstructive Surgery the development of new innovative matrices for skin repair is in urgent need. The ideal biomaterial should promote attachment, proliferation and growth of cells. Additionally, it should degrade in an appropriate time period without releasing harmful substances, but not exert a pathological immune response. Spider dragline silk from Nephila spp meets these demands to a large extent. Methodology/Principal Findings: Native spider dragline silk, harvested directly out of Nephila spp spiders, was woven on steel frames. Constructs were sterilized and seeded with fibroblasts. After two weeks of cultivating single fibroblasts, keratinocytes were added to generate a bilayered skin model, consisting of dermis and epidermis equivalents. For the next three weeks, constructs in co-culture were lifted on an originally designed setup for air/liquid interface cultivation. After the culturing period, constructs were embedded in paraffin with an especially developed program for spidersilk to avoid supercontraction. Paraffin c