atlantic salmon reovirus infection causes a cd8 t cell myocarditis in atlantic salmon (salmo salar l.)大西洋鲑鱼呼肠孤病毒感染导致大西洋鲑鱼cd8 t细胞心肌炎(大西洋鲑l .).pdfVIP

Atlantic Salmon Reovirus Infection Causes a CD8 T Cell Myocarditis in Atlantic Salmon (Salmo salar L.) 1 1 2 2 1 Aase B. Mikalsen , Oyvind Haugland , Marit Rode , Inge Tom Solbakk , Oystein Evensen * 1 Department of Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary Science, Oslo, Norway, 2 PHARMAQ AS, Oslo, Norway Abstract Heart and skeletal inflammation (HSMI) of farmed Atlantic salmon (Salmo salar L.) is a disease characterized by a chronic myocarditis involving the epicardium and the compact and spongious part of the heart ventricle. Chronic myositis of the red skeletal muscle is also a typical finding of HSMI. Piscine reovirus (PRV) has been detected by real-time PCR from farmed and wild salmon with and without typical changes of HSMI and thus the causal relationship between presence of virus and the disease has not been fully determined [1]. In this study we show that the Atlantic salmon reovirus (ASRV), identical to ¨ PRV, can be passaged in GF-1 cells and experimental challenge of naıve Atlantic salmon with cell culture passaged reovirus results in cardiac and skeletal muscle pathology typical of HSMI with onset of pathology from 6 weeks, peaking by 9 weeks post challenge. ASRV replicates in heart tissue and the peak level of virus replication coincides with peak of heart lesions. We further demonstrate mRNA transcript assessment and in situ characterization that challenged fish develop a CD8+ T cell myocarditis. Citation: Mikalsen AB, Haugland O, Rode M, Solbakk IT, Evensen O (2012) Atlantic Salmon Reovirus Infection Causes a CD8 T Cell Myocarditis in Atlantic Salmon (Salmo salar L.). PLoS ONE 7(6): e37269. doi:10.1371/j