ascaroside expression in caenorhabditis elegans is strongly dependent on diet and developmental stageascaroside表达线虫强烈依赖于饮食和发展阶段.pdfVIP

Ascaroside Expression in Caenorhabditis elegans Is Strongly Dependent on Diet and Developmental Stage 1 2 3 4 2 Fatma Kaplan , Jagan Srinivasan , Parag Mahanti , Ramadan Ajredini , Omer Durak , Rathika 3 2 1 3 4 Nimalendran , Paul W. Sternberg , Peter E. A. Teal , Frank C. Schroeder , Arthur S. Edison , Hans T. Alborn1* 1 Center for Medical, Agricultural and Veterinary Entomology, USDA-ARS, Gainesville, Florida, United States of America, 2 Medical Institute and Biology Division, California Institute of Technology, Pasadena, California, United States of America, 3 Boyce Thompson Institute and Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York, United States of America, 4 Department of Biochemistry and Molecular Biology, High Magnetic Field Laboratory, University of Florida, Gainesville, Florida, United States of America Abstract Background: The ascarosides form a family of small molecules that have been isolated from cultures of the nematode Caenorhabditis elegans. They are often referred to as ‘‘dauer pheromones’’ because most of them induce formation of long- lived and highly stress resistant dauer larvae. More recent studies have shown that ascarosides serve additional functions as social signals and mating pheromones. Thus, ascarosides have multiple functions. Until now, it has been generally assumed that ascarosides are constitutively expressed during nematode development. Methodology/Principal Findings: Cultures of C. elegans were developmentally synchronized on controlled diets. Ascarosides released into