ascorbate peroxidase from leishmania major controls the virulence of infective stage of promastigotes by regulating oxidative stress抗坏血酸盐过氧化物酶从利什曼虫感染性阶段的主要控制毒性promastigotes通过调节氧化应激.pdfVIP

Ascorbate Peroxidase from Leishmania major Controls the Virulence of Infective Stage of Promastigotes by Regulating Oxidative Stress Swati Pal, Subhankar Dolai, Rajesh K. Yadav, Subrata Adak* Division of Structural Biology Bio-Informatics, Indian Institute of Chemical Biology, Kolkata, West Bengal, India Abstract Background: Peroxidase represents a heterogeneous group of distinct enzyme family that plays extremely diverse biological functions. Ascorbate peroxidase from Leishmania major (LmAPX) has been shown to be central to the redox defense system of Leishmania. To investigate further its exact physiological role in Leishmania, we attempted to create LmAPX -knockout mutants by gene replacement in L. major strains. Methodology/Principal Findings: The null mutant cell culture contains a higher percentage of metacyclic and apoptotic cells compared to both wild type and LmAPX overexpressing cells. Flowcytometric analysis reveals the presence of a higher concentration of intracellular H O , indicative of increased oxidative stress in parasites lacking LmAPX. IC value for 2 2 50 exogenously added H O shows that deletion of LmAPX in L. major renders the cell more susceptible to H O . Real time PCR 2 2 2 2 studies demonstrate an elevated mRNA level of non-selenium glutathione peroxidase in LmAPX null mutant cell line, suggesting that these enzymes were induced to compensate the LmAPX enzyme. The null mutant cells exhibit hypervirulence after in