atomic force microscopy analysis of the acinetobacter baumannii bacteriophage ap22 lytic cycle原子力显微镜分析鲍曼不动杆菌噬菌体ap22裂解周期.pdfVIP

Atomic Force Microscopy Analysis of the Acinetobacter baumannii Bacteriophage AP22 Lytic Cycle 1,2 . 3. 4 3 Evgeniy V. Dubrovin * , Anastasia V. Popova , Sergey V. Kraevskiy , Sergei G. Ignatov , Tatyana E. Ignatyuk4, Igor V. Yaminsky1,2, Nikolay V. Volozhantsev3 1 M.V. Lomonosov Moscow State University, Moscow, Russian Federation, 2 Advanced Technologies Center, Moscow, Russian Federation, 3 State Research Center for Applied Microbiology and Biotechnology, Obolensk, Russian Federation, 4 Alikhanov Institute for Theoretical and Experimental Physics, Moscow, Russian Federation Abstract Background: Acinetobacter baumannii is known for its ability to develop resistance to the major groups of antibiotics, form biofilms, and survive for long periods in hospital environments. The prevalence of infections caused by multidrug-resistant A. baumannii is a significant problem for the modern health care system, and application of lytic bacteriophages for controlling this pathogen may become a solution. Methodology/Principal Findings: In this study, using atomic force microscopy (AFM) and microbiological assessment we have investigated A. baumannii bacteriophage AP22, which has been recently described. AFM has revealed the morphology of bacteriophage AP22, adsorbed on the surfaces of mica, graphite and host bacterial cells. Besides, morphological changes of bacteriophage AP22-infected A. baumannii cells were characterized at different stages of the lytic cycle, from phage adsorption to the cell lysis. The phage latent period, estimated from AFM was in good agreement with that obtained by microbiological methods (40 min). Bacteriophage AP22, whose head diameter is 6261 nm and tail length is 8869