atmospheric oxygen tension slows myoblast proliferation via mitochondrial activation通过激活线粒体大气氧含量降低成肌细胞增殖.pdfVIP

Atmospheric Oxygen Tension Slows Myoblast Proliferation via Mitochondrial Activation 1,2. 1,2 . 1 1 1 Stephanie Duguez , William J. Duddy * , Viola Gnocchi , James Bowe , Sherry Dadgar , Terence A. Partridge1 ´ 1 Research Center for Genetic Medicine, Children’s National Medical Center, Washington, District of Columbia, United States of America, 2 Universite Pierre et Marie Curie ´ ´ (UPMC UMR S 974)-Institut National de la Sante et de la Recherche Medicale (Inserm U974)-Centre National de la Recherche Scientifique (CNRS UMR 7215), Institut de Myologie, Paris, France Abstract Background: Mitochondrial activity inhibits proliferation and is required for differentiation of myoblasts. Myoblast proliferation is also inhibited by the ,20% oxygen level used in standard tissue culture. We hypothesize that mitochondrial activity would be greater at hyperoxia (20% O ) relative to more physiological oxygen (5% O ). 2 2 Methodology/Principal Findings: Murine primary myoblasts from isolated myofibres and conditionally immortalized H-2K myoblasts were cultured at 5% and 20% oxygen. Proliferation, assayed by cell counts, EdU labeling, and CFSE dilution, was slower at 20% oxygen. Expression of MyoD in primary myoblasts was delayed at 20% oxygen, but myogenicity, as measured by fusion index, was slightly higher. FACS-bas