原创力文档characterizing the escherichia coli o157h7 proteome including protein associations with higher order assemblies描述蛋白质组e大肠杆菌o157h7包括蛋白质对高阶组件.pdfVIP
- 1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
- 2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
characterizing the escherichia coli o157h7 proteome including protein associations with higher order assemblies描述蛋白质组e大肠杆菌o157h7包括蛋白质对高阶组件
Characterizing the Escherichia coli O157:H7 Proteome
Including Protein Associations with Higher Order
Assemblies
1 2 1 1 1
Rembert Pieper *, Quanshun Zhang , David J. Clark , Shih-Ting Huang , Moo-Jin Suh , John C.
1 1 1 1 2
Braisted , Samuel H. Payne , Robert D. Fleischmann , Scott N. Peterson , Saul Tzipori
1J. Craig Venter Institute, Rockville, Maryland, United States of America, 2 Division of Infectious Diseases, Cummings School of Veterinary Medicine, Tufts University, North
Grafton, Massachusetts, United States of America
Abstract
Background: The recent outbreak of severe infections with Shiga toxin (Stx) producing Escherichia coli (STEC) serotype
O104:H4 highlights the need to understand horizontal gene transfer among E. coli strains, identify novel virulence factors
and elucidate their pathogenesis. Quantitative shotgun proteomics can contribute to such objectives, allowing insights into
the part of the genome translated into proteins and the connectivity of biochemical pathways and higher order assemblies
of proteins at the subcellular level.
Methodology/Principal Findings: We examined protein profiles in cell lysate fractions of STEC strain 86-24 (serotype
O157:H7), following growth in cell culture or bacterial isolation from intestines of infected piglets, in the context of
functionally and structurally characterized biochemical pathways of E. coli. Protein solubilization in the presence of Triton X-
100, EDTA and high salt was followed by size exclusion chromatography into the approximate Mr ranges greater than
280 kDa, 280-80 k
您可能关注的文档
- cdk1 targets srs2 to complete synthesis-dependent strand annealing and to promote recombinational repaircdk1目标srs2 synthesis-dependent链退火,促进重组修复完成.pdf
- cdk4 regulates recruitment of quiescent β-cells and ductal epithelial progenitors to reconstitute β-cell mass到调节静β-cells招聘和导管上皮细胞祖细胞重组β-cell质量.pdf
- cdk2 is required for p53-independent g2m checkpoint controlcdk2需要p53-independent g2m检查点控制.pdf
- cdk5 is required for memory function and hippocampal plasticity via the camp signaling pathwaycdk5需要记忆功能和海马可塑性通过信号通路.pdf
- cd133 (prominin) negative human neural stem cells are clonogenic and tripotentcd133(prominin)-人类神经干细胞克隆细胞和tripotent.pdf
- cdk11p58 is required for centriole duplication and plk4 recruitment to mitotic centrosomes需要cdk11p58中心体复制和plk4招聘有丝分裂中心体.pdf
- cebpβ promotes transition from proliferation to hypertrophic differentiation of chondrocytes through transactivation of p57kip2cebpβ促进从扩散通过transactivation p57kip2的肥厚性软骨细胞的分化.pdf
- cdkn1c (p57kip2) is a direct target of ezh2 and suppressed by multiple epigenetic mechanisms in breast cancer cellscdkn1c(p57kip2)是一种直接的目标ezh2和抑制由多个表观遗传机制在乳腺癌细胞.pdf
- cdc20 is critical for meiosis i and fertility of female micecdc20减数分裂我和生育能力的雌性老鼠是至关重要的.pdf
- cdk5 is essential for soluble amyloid β-induced degradation of gkap and remodeling of the synaptic actin cytoskeletoncdk5至关重要的可溶性淀粉样β-induced gkap退化和突触肌动蛋白细胞骨架的改建.pdf
- characterizing the host and symbiont proteomes in the association between the bobtail squid, euprymna scolopes, and the bacterium, vibrio fischeri特征之间的关联的主机和共生有机体蛋白质组短尾猫鱿鱼,euprymna scolopes,,费氏弧菌和.pdf
- characterization of x-ray dose in murine animals using microct, a new low-dose detector and nanodot dosimeters使用microct特征x射线剂量的小鼠动物,一个新的低剂量探测器和nanodot测试仪.pdf
- characterizing acupuncture stimuli using brain imaging with fmri - a systematic review and meta-analysis of the literature描述针灸刺激使用大脑成像和功能磁共振成像,文献的系统回顾和荟萃分析.pdf
- characterizing the network of drugs and their affected metabolic subpathways描述网络影响的药物及其代谢subpathways.pdf
- characterizing the metabolism of dehalococcoides with a constraint-based model与基于模型描述dehalococcoides的新陈代谢.pdf
- charting the interplay between structure and dynamics in complex networks绘制复杂网络的结构和动力学之间的相互作用.pdf
- charge as a selection criterion for translocation through the nuclear pore complex作为选择的标准收取通过核孔复杂易位.pdf
- charge profile analysis reveals that activation of pro-apoptotic regulators bax and bak relies on charge transfer mediated allosteric regulation负责资料分析表明,激活pro-apoptotic监管者伯灵顿和贝克依靠电荷转移变构调节介导的.pdf
- characterizing tumor response to chemotherapy at various length scales using temporal diffusion spectroscopy描述肿瘤化疗反应在不同使用时间扩散光谱长度尺度.pdf
- charting the spread of salmonella infection图表沙门氏菌感染的传播.pdf
文档评论（0）