chromatin regulation and gene centrality are essential for controlling fitness pleiotropy in yeast染色质调节和基因中心健身多向性酵母控制至关重要.pdfVIP

Chromatin Regulation and Gene Centrality Are Essential for Controlling Fitness Pleiotropy in Yeast 1 2 1 1,3 Linqi Zhou , Xiaotu Ma , Michelle N. Arbeitman , Fengzhu Sun * 1 Department of Biological Sciences, University of Southern California, Los Angeles, California, United States of America, 2 Department of Molecular and Cell Biology, University of Texas at Dallas, Richardson, Texas, United States of America, 3 MOE Key Laboratory of Bioinformatics and Bioinformatics Division, TNLIST/Department of Automation, Tsinghua University, Beijing, People’s Republic of China Abstract Background: There are a wide range of phenotypes that are due to loss-of-function or null mutations. Previously, the functions of gene products that distinguish essential from nonessential genes were characterized. However, the functions of products of non-essential genes that contribute to fitness remain minimally understood. Principal Findings: Using data from Saccharomyces cerevisiae, we investigated several gene characteristics, which we are able to measure, that are significantly associated with a gene’s fitness pleiotropy. Fitness pleiotropy is a measurement of the gene’s importance to fitness. These characteristics include: 1) whether the gene’s product functions in chromatin regulation, 2) whether the regulation of the gene is influenced by chromatin state, measured by chromatin regulation effect (CRE), 3) whether the gene’s product functions as a transcription factor (TF) and the number of genes a TF regulates, 4) whether the gene contains TATA-box, and 5) whether the gene’s product is central in a protein interaction network. Partial correlation analysis was used to study how these characteristics interact to influence fitness plei