cthrc1 is a positive regulator of osteoblastic bone formationcthrc1成骨细胞的骨形成的是一个积极的监管机构.pdfVIP

Cthrc1 Is a Positive Regulator of Osteoblastic Bone Formation 1 2,4 2 2 3 Hiroaki Kimura , Kin Ming Kwan , Zhaoping Zhang , Jian Min Deng , Bryant G. Darnay , Richard R. 2 1 2 1 Behringer , Takashi Nakamura , Benoit de Crombrugghe , Haruhiko Akiyama * 1 Department of Orthopaedics, Kyoto University, Kyoto, Japan, 2 Department of Molecular Genetics, The University of Texas M. D. Anderson Cancer Center, Houston, Texas, United States of America, 3 Department of Experimental Therapeutics, The University of Texas M. D. Anderson Cancer Center, Houston, Texas, United States of America, 4 Department of Biology, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China Abstract Background: Bone mass is maintained by continuous remodeling through repeated cycles of bone resorption by osteoclasts and bone formation by osteoblasts. This remodeling process is regulated by many systemic and local factors. Methodology/Principal Findings: We identified collagen triple helix repeat containing-1 (Cthrc1) as a downstream target of bone morphogenetic protein-2 (BMP2) in osteochondroprogenitor-like cells by PCR-based suppression subtractive hybridization followed by differential hybridization, and found that Cthrc1 was expressed in bone tissues in vivo. To investigate the role of Cthrc1 in bone, we generated Cthrc1-null mice and transgenic mice which overexpress Cthrc1 in osteoblasts (Cthrc1 transgenic mice). Microcomputed tomography (micro-CT) and bone histomorphometry analyses showed th