defective secretion of islet hormones in chromogranin-b deficient mice缺陷在chromogranin-b缺陷小鼠胰岛激素的分泌.pdfVIP

Defective Secretion of Islet Hormones in Chromogranin- B Deficient Mice ¨ 1. 2,3.¤ 4 1 1 Stefanie Obermuller , Federico Calegari , Angus King , Anders Lindqvist , Ingmar Lundquist , 1 3 3 5 2,4 Albert Salehi , Maura Francolini , Patrizia Rosa , Patrik Rorsman , Wieland B. Huttner *, Sebastian Barg1,6* ¨ ¨ 1 Department of Clinical Sciences-Malmo, Lund University, Malmo, Sweden, 2 Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany, 3 CNR Institute of Neuroscience, Department of Medical Pharmacology, University of Milan, Milan, Italy, 4 Department of Neurobiology, University of Heidelberg, Heidelberg, Germany, 5 Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), University of Oxford, Churchill Hospital, Oxford, United Kingdom, 6 Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden Abstract Granins are major constituents of dense-core secretory granules in neuroendocrine cells, but their function is still a matter of debate. Work in cell lines has suggested that the most abundant and ubiquitously expressed granins, chromogranin A and B (CgA and CgB), are involved in granulogenesis and protein sorting. Here we report the generation and characterization of mice lacking chromogranin B (CgB-ko), which were viable and fertile. Unlike neuroendocrine tissues, pancreatic islets of