dengue 1 diversity and microevolution, french polynesia 2001–2006 connection with epidemiology and clinics登革1多样性和微进化,法属波利尼西亚2001 - 2006与流行病学和诊所.pdfVIP

Dengue 1 Diversity and Microevolution, French Polynesia 2001–2006: Connection with Epidemiology and Clinics 1,2 2 2 1 Elodie Descloux *, Van-Mai Cao-Lormeau , Claudine Roche , Xavier De Lamballerie ´ ´ ´ ´ 1 UMR190, Emergence des Pathologies Virales, Universite de la Mediterranee and Institut de Recherche pour le Developpement, Marseille, France, 2 Laboratoire de ´ ´ Recherche en Virologie Medicale, Institut Louis Malarde, Papeete, Tahiti, French Polynesia Abstract Background: Dengue fever (DF) is an emerging infectious disease in the tropics and subtropics. Determinants of DF epidemiology and factors involved in severe cases—dengue haemorrhagic fever (DHF) and dengue shock syndrome (DSS)—remain imperfectly characterized. Since 2000, serotype 1 (DENV-1) has predominated in the South Pacific. The aim of this study was (i) to determine the origin and (ii) to study the evolutionary relationships of DENV-1 viruses that have circulated in French Polynesia (FP) from the severe 2001 outbreak to the recent 2006 epidemic, and (iii) to analyse the viral intra-host genetic diversity according to clinical presentation. Methodology/Principal Findings: Sequences of 181 envelope gene and 12 complete polyproteins of DENV-1 viruses obtained from human sera in FP during the 2001–2006 period were generated. Phylogenetic analysis showed that all DENV- 1 FP strains belonged to genotype IV–‘‘South Pacific’’ and derived from a single introduction event from South-East Asia followed by a 6-year in situ evolution. Althoug