原创力文档determination of diniconazole in agricultural samples by sol-gel immunoaffinity extraction procedure coupled with hplc and elisa测定diniconazole农业样本通过溶胶-凝胶法immunoaffinity萃取过程耦合与高效液相色谱法和elisa.pdfVIP
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determination of diniconazole in agricultural samples by sol-gel immunoaffinity extraction procedure coupled with hplc and elisa测定diniconazole农业样本通过溶胶-凝胶法immunoaffinity萃取过程耦合与高效液相色谱法和elisa
Determination of Diniconazole in Agricultural Samples
by Sol-Gel Immunoaffinity Extraction Procedure Coupled
with HPLC and ELISA
Zhenjiang Liu, Yahui Jin, Minghua Wang*
Department of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Jiangsu Key Laboratory of Pesticide Science, Key Laboratory of Integrated
Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China
Abstract
Background: In the European Union (EU), the use of diniconazole-M is no longer authorized. However, residues of
diniconazole-M occur in various plant commodities.
Methodology/Principal Findings: A selective and simple analytical method for the trace level determination of diniconazole
in soil, fruit, vegetables and water samples was developed based on immunoaffinity extraction followed by Enzyme-linked
immunosorbent assay (ELISA) and the high-performance liquid chromatography (HPLC) analysis. The ELISA was based on
monoclonal antibodies highly specific to diniconazole and was a fast, cost-effective, and selective screening method for the
detection of diniconazole. The results of the ELISA correlated well with gas chromatography (GC) results, with the
correlation coefficient of 0.9879 (n = 19). A simple gel permeation chromato- graphy clean-up method was developed to
purify extracts from matrices containing high amounts of fat and natural pigments, without the need for a large dilution of
the sample. The immunoaffinity column (IAC) capacity was 0.180 mg g21. The columns could be re-used approximately 20
times with no significant alteration in capacity. The recoveries from complex samples were in the range of 89.2% to 96.1%
with a relative standard deviation (RSD) of 0.770%–6.11% by ELISA. The results were in good agreement with those
obtained by HPLC method.
Conclusion/Sig
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