原创力文档differential expression of carotenogenic genes, associated changes on astaxanthin production and photosynthesis features induced by ja in h. pluvialis微分carotenogenic基因的表达,相关联的变化虾青素生产和ja诱导的h . pluvialis光合作用特性.pdfVIP
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differential expression of carotenogenic genes, associated changes on astaxanthin production and photosynthesis features induced by ja in h. pluvialis微分carotenogenic基因的表达,相关联的变化虾青素生产和ja诱导的h . pluvialis光合作用特性
Differential Expression of Carotenogenic Genes,
Associated Changes on Astaxanthin Production and
Photosynthesis Features Induced by JA in H. pluvialis
2. 2. 3 3 2 4
Zhengquan Gao , Chunxiao Meng , Xiaowen Zhang , Dong Xu , Yuefeng Zhao , Yitao Wang ,
2 2 1 3
Hongxin Lv , Liming Yang , Lingling Chen *, Naihao Ye *
1 State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China, 2 School of Life
Sciences, Shandong University of Technology, Zibo, Shandong, China, 3 Yellow Sea Fishery Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong,
China, 4 Qingdao Agricultural University, Qingdao, Shandong, China
Abstract
Haematococcus pluvialis is an organism that under certain conditions can produce astaxanthin, an economically important
carotenoid. In this study, the transcriptional expression patterns of eight carotenogenic genes of H. pluvialis in response to
jasmonic acid (JA) were evaluated using real-time PCR. Astaxanthin accumulation action and photosynthesis flourescence
were monitored at the same time. The results showed all eight genes exhibited higher transcriptional expression
significantly under JA treatments. JA25 (25 mg/L) induction had greater effect (.10-fold up-regulation) on the
transcriptional expression of pds, crtR-B and lyc than on ipi-1, ipi-2, psy, bkt2, and crtO. JA50 (50 mg/L) treatment had
greater impact on the transcriptional expression of ipi-1, ipi-2, psy, crtR-B and crtO than on pds, lyc and bkt2. Astaxanthin
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