differential expression of wnts after spinal cord contusion injury in adult rats微分表达式实际上wnt在成年大鼠脊髓挫伤后受伤.pdfVIP

Differential Expression of Wnts after Spinal Cord Contusion Injury in Adult Rats ´ ´ 1 ´ ´ 1 ´ 1 1 Carmen Marıa Fernandez-Martos , Carlos Gonzalez-Fernandez , Pau Gonzalez , Alfredo Maqueda , 2 ´ 1 Ernest Arenas , Francisco Javier Rodrıguez * ´ ´ 1 Laboratorio de Neurologıa Molecular, Hospital Nacional de Paraplejicos (HNP), Toledo, Spain, 2 Molecular Neurobiology Unit, MBB, Karolinska Institute, Stockholm, Sweden Abstract Background: Spinal cord injury is a major cause of disability that has no clinically accepted treatment. Functional decline following spinal cord injury is caused by mechanical damage, secondary cell death, reactive gliosis and a poor regenerative capacity of damaged axons. Wnt proteins are a family of secreted glycoproteins that play key roles in different developmental processes although little is known of the expression patterns and functions of Wnts in the adult central nervous system in normal or diseased states. Findings: Using qRT-PCR analysis, we demonstrate that mRNA encoding most Wnt ligands and soluble inhibitors are constitutively expressed in the healthy adult spinal cord. Strikingly, contusion spinal cord injury induced a time-dependent increase in Wnt mRNA expression from 6 hours until 28 days post-injury, and a narrow peak in the expression of soluble Wnt inhibitors between 1 and 3 days post-injury. These results are consistent with the increase in the migration shift, from day 1 to 7, of the intracellular Wnt signalling component, Dishevelled-3. Moreover, after an initial decr