differential gene expression at coral settlement and metamorphosis - a subtractive hybridization study差异基因表达在珊瑚结算和蜕变u2014u2014一个减法杂交研究.pdfVIP

Differential Gene Expression at Coral Settlement and Metamorphosis - A Subtractive Hybridization Study 1 1,2 2 1,3 ˆ 3 David C. Hayward , Suzannah Hetherington , Carolyn A. Behm , Lauretta C. Grasso , Sylvain Foret , 3 1 David J. Miller , Eldon E. Ball * 1 Evolution, Ecology and Genetics, Research School of Biology, Australian National University, Canberra, Australian Capital Territory, Australia, 2 Biomedical Science and Biochemistry, Research School of Biology, Australian National University, Canberra, Australian Capital Territory, Australia, 3 ARC Centre of Excellence for Coral Reef Studies and School of Pharmacy and Molecular Sciences, James Cook University, Townsville, Queensland, Australia Abstract Background: A successful metamorphosis from a planktonic larva to a settled polyp, which under favorable conditions will establish a future colony, is critical for the survival of corals. However, in contrast to the situation in other animals, e.g., frogs and insects, little is known about the molecular basis of coral metamorphosis. We have begun to redress this situation with previous microarray studies, but there is still a great deal to learn. In the present paper we have utilized a different technology, subtractive hybridization, to characterize genes differentially expressed across this developmental transition and to compare the success of this method to microarray. Methodology/Principal Findings: Suppressive subtractive hybridization (SSH) was used to identify two pools of transcripts from the coral, Acropora millepora. One is enriched for transcripts expressed at higher levels at the pre-settlement sta