effect of acute plasmodium falciparum malaria on reactivation and shedding of the eight human herpes viruses急性恶性疟原虫疟疾对复活的影响和人类疱疹病毒8脱落.pdfVIP

Effect of Acute Plasmodium falciparum Malaria on Reactivation and Shedding of the Eight Human Herpes Viruses ˆ 1 ´ 1 2 1,2 3 Arnaud Chene , Susanne Nylen , Daria Donati , Maria Teresa Bejarano , Fred Kironde , Mats Wahlgren1*, Kerstin I. Falk1,4 1 Department of Microbiology Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Sweden, 2 Department of Medicine, Center for Infectious Medicine, Karolinska Institutet, Stockholm, Sweden, 3 Department of Biochemistry, Faculty of Medicine, Makerere University, Kampala, Uganda, 4 Swedish Institute for Communicable Disease Control, Solna, Sweden Abstract Human herpes viruses (HHVs) are widely distributed pathogens. In immuno-competent individuals their clinical outcomes are generally benign but in immuno-compromised hosts, primary infection or extensive viral reactivation can lead to critical diseases. Plasmodium falciparum malaria profoundly affects the host immune system. In this retrospective study, we evaluated the direct effect of acute P. falciparum infection on reactivation and shedding of all known human herpes viruses (HSV-1, HSV-2, VZV, EBV, CMV, HHV-6, HHV-7, HHV-8). We monitored their presence by real time PCR in plasma and saliva of Ugandan children with malaria at the day of admission to the hospital (day-0) and 14 days later (after treatment), or in children with mild infections unrelated to malaria. For each child screened in this study, at least one type of HHV was detected in the saliva. HHV-7 and HHV-6 were detected in more than 70% of the samples and CMV in approximately half. HSV-1, HSV-2, VZV and HHV-8 were detected at lower frequency. During salivary shedd