screen-printed carbon electrodes modified by rhodium dioxide and glucose dehydrogenase丝网印刷碳电极改性铑二氧化碳和葡萄糖脱氢酶.pdfVIP

SAGE-Hindawi Access to Research Enzyme Research Volume 2010, Article ID 324184, 7 pages doi:10.4061/2010/324184 Research Article Screen-Printed Carbon Electrodes Modified by Rhodium Dioxide and Glucose Dehydrogenase ˇ ˇ Vojtech Polan, Jan Soukup, and Karel Vytras Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentska 573, ´ 532 10 Pardubice, Czech Republic ˇ Correspondence should be addressed to Karel Vytras, karel.vytras@upce.cz Received 3 June 2010; Accepted 15 December 2010 Academic Editor: Raffaele Porta ˇ Copyright © 2010 Vojtech Polan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The described glucose biosensor is based on a screen-printed carbon electrode (SPCE) modified by rhodium dioxide, which functions as a mediator. The electrode is further modified by the enzyme glucose dehydrogenase, which is immobilized on the electrode’s surface through electropolymerization with m-phenylenediamine. The enzyme biosensor was optimized and tested in model glucose samples. The biosensor showed a linear range of 500–5000 mg L−1 of glucose with a detection limit of 210 mg L−1 (established as 3σ ) and response time of 39 s. When compared with similar glucose biosensors based on glucose oxidase, the main advantage is that neither ascorbic and uric acids nor paracetamol interfere measurements