suppressive effect of juzen-taiho-to on lung metastasis of b16 melanoma cells in vivo抑制黑色素瘤b16转椅的juzen-taiho-to对肺转移的影响细胞体内.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2011, Article ID 743153, 5 pages doi:10.1093/ecam/nen081 Original Article Suppressive Effect of Juzen-Taiho-To on Lung Metastasis of B16 Melanoma Cells In Vivo Takako Matsuda, Katsuhiko Maekawa, Kazuhito Asano, and Tadashi Hisamitsu Department of Physiology, School of Medicine, Showa University, Tokyo, Japan Correspondence should be addressed to Takako Matsuda, mtakako@y4.dion.ne.jp Received 14 February 2008; Accepted 27 November 2008 Copyright © 2011 Takako Matsuda et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Juzen-Taiho-To (JTT) is well known to be one of Kampo (Japanese herbal) medicine consisted of 10 component herbs and used for the supplemental therapy of cancer patients with remarkably success. However, the precise mechanisms by which JTT could favorably modify the clinical conditions of cancer patients are not well defined. The present study, therefore, was undertaken to examine the possible mechanisms of JTT on prevention of cancer metastasis using experimental mouse model. JTT was well mixed with rodent chow at concentrations of either 0.2 or 1.0%, and administered orally ad libitum, which was started 1 week before tumor cell injection and continue throughout the experiment. Oral administration of JTT at concentration 0.2 and 1.0% into C57BL/6 male mice significantly inhibited tumor metastasis in lungs, which was induced by the intravenous injection of 2 × 105 B16 melanoma cell. JTT at a concentration of 1.0% also significantly suppressed lung metastasis of B16 melanoma cell from hind fo