the association of dimethylsulfoxide and model membranes studied by pulse-field gradient nmr二甲亚砜和模型研究了膜协会pulse-field梯度核磁共振.pdfVIP

Spectroscopy 18 (2004) 265–269 265 IOS Press The association of dimethylsulfoxide and model membranes studied by pulse-field gradient NMR 1 Anthony Kennedy a,∗, Carla J. Long a , Peter J. Hmel a , Rickey Hicks b and Thomas J. Reid a a Department of Blood Research, Division of Military Casualty Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA b Department of Medicinal Chemistry, Division of Experimental Therapeutics, Walter Reed Army Institute of Research, Silver Spring, MD, USA Abstract. The use of dimethylsulfoxide (DMSO) as a cryoprotectant to reduce cellular injury during freezing is well known, however the intermolecular interactions between this amphiphilic molecule and biological membranes that form the basis of this protection are unknown. DMSO–dipalmitoylphosphatidylcholine (DPPC) vesicle interactions were investigated in pulsed- field gradient NMR (PFGNMR) experiments and spectra analysis allowed for the determination of self-diffusion coefficients for each species present. The mole fraction of DMSO associated with the DPPC vesicles was then calculated from the diffusion coefficients: the mole fraction increased from 14% to 42% as the membrane was heated from below to above the main phase transition temperature. 1. Introduction The study of the interactions of cryoprotectants with cell membranes has become increasingly impor- tant because of the expanding need for long-term cryopreservation of cells. An important cryoprotectant widely used in cell cryopreservation is the amphiphilic molecule dimethylsulfoxide (DMSO). The ability of DMSO to protect cells during freezing and reduce cellu- lar damage is well known [1–9]. DMSO appears to protect cells from freezing induced injury not only by influencing extracellular and intracellular ice crystal formation but also through exerting a protective