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Thickness variations within endothelial grafts for DSAEK 厚度变化范围内的内皮移植角膜内皮移植术
Thickness variations within endothelial grafts for DSAEK surgery. A spectral domain anterior segment OCT study Alberto Neri, Rosachiara Leaci, Patrizia Scaroni, Claudio Macaluso Ophthalmology, University of Parma, Italy The authors have no financial interest in the subject matter of this poster DSAEK Descemet Stripping Automated Endothelial Keratoplasty Currently the standard procedure for endothelial pathology. Surgical procedure: Descemet stripping Insertion of the endothelial graft Adesion with air bubble Microkeratome (or femtosecond laser) cut: asportation of a 300 / 350 / 400 μm anterior lamella Donor button harvesting: Trephination The normal cornea is thicker in the periphery and thinner in the center. Tissue harvesting with microkeratome and femtolaser, that measure lamellar thickness from epithelial surface, creates regular lamellae for anterior keratoplasty techniques, but the remaining endothelial lamellae, that are the one used for DSAEK, necessarily change in thickness from center to periphery. Cross sectional profile = Meniscus PURPOSE To evaluate variations in thickness of endothelial grafts for DSAEK surgery dependent on both eccentricity and meridian orientation using high resolution anterior segment OCT (CASIA?, Tomey, Japan). MATERIALS and METHODS 20 eyes of 20 patients who underwent DSAEK surgery were examined. Full anterior segment scans were captured with CASIA? OCT. The optical centre of the cornea and eight meridians (0°, 45°, 90°, 135°, 180°, 225°, 270° and 315°) were analyzed with the FLAP TOOL, that allowed precise identification and measurement of endothelial graft thickness. Flap thickness was measured at the center and at 1,5mm and 3mm eccentricity along each meridian. ANALYSIS DATA for each analyzed eye (20): Central flap thickness 8 thickness values at 1.5 mm eccentricity 8 thickness values at 3mm eccentricity Descriptive analysis of thickness values was performed for each eccentricity. To describe the variation in t
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