原核生物DNA复制中的酶和蛋白质-2.PPT

Prepriming involves formation of a complex by sequential association of proteins, leading to the separation of DNA strands. methylation at the origin A membrane-bound inhibitor binds to hemimethylated DNA at the origin, and may function by preventing the binding of DnaA. It is released when the DNA is remethylated. SeqA The complex at oriC can be detected by electron microscopy. Antibodies of dnaA protein HU The protein HU is a general DNA-binding protein in E. coli . Its presence is not absolutely required to initiate replication in vitro, but it stimulates the reaction. HU has the capacity to bend DNA, and is likely to be involved in some general structural capacity. 2、参与复制延伸的蛋白质因子 (Dna G) (DnaB) 2、参与复制终止的蛋白质因子 Tus * * 参与原核生物DNA复制的 酶类和蛋白质 Enzymes and Proteins Involved in DNA Replication in Prokaryotes 高方远 马欣荣 康海岐 DNA replication(bacteria) Initiation Elongation Termination Daughter DNA partition * the origin of replication is defined * replication bubble. * Replication fork * Unidirectional or bidirectional. Origins Figure 13.9 The leading strand is synthesized continuously while the lagging strand is synthesized discontinuously. Elongation(semidiscontinuous) termination 参与DNA复制的酶类 1、DNA聚合酶 2、DNA引发酶（DNA primase） 3、DNA连接酶 4、与DNA几何学性质相关的酶 I polA major repair enzyme II polB minor repair enzyme III polC replicase IV dinB SOS repair V umuC、D SOS repair DNA polymerases in E.coli DNA Polymerase I DNA polymerase 3’ 5’ exonuclease 5’ 3’ exonuclease Figure 13.8 The catalytic domain of a DNA polymerase has a DNA-binding cleft created by three subdomains. The active site is in the palm. Proofreading is provided by a separate active site in an exonuclease domain. Figure 13.7 Crystal structure of phage T7 DNA polymerase has a right hand structure. DNA lies across the palm and is held by the fingers and thumb. Photograph kindly provided by Charles Richardson and Tom Ellenberger.