GAS CHROMATOGRAPHY Indian Institute of （气相色谱的印度理工学院）.pdfVIP

PI 306 GAS CHROMATOGRAPHY AIM To study the Gas Chromatography, study of influence of various operating parameters on the performance of GC. To analyze the sample of unknown concentration using Gas Chromatography. APPARATUS Gas Chromatograph, GC analyzer, normal syringes and one micro syringe, Beakers, Sample bottles and Electronic weight. CHEMICALS Methanol, Isopropyl Alcohol and water THEORY A Gas Chromatograph is used to detect the components based on the selective affinity of components towards the adsorbent materials. The sample is introduced in the liquid/gas form with the help of GC syringe into the injection port, it gets vaporized at injection port then passes through column with the help of continuously flowing carrier stream (mobile phase), mainly H2 (for TCD), and gets separated/detected at the detection port with suitable temperature programming. We visualize this on computer in the form of peaks. Carrier medium can be liquid (e.g. HPLC) or gas (e.g. GC) for the ease of separation/detection, if it is gas then called gas chromatography otherwise called liquid chromatography. Different chemical constituents of the sample travel through the column at different rates depending upon, 1. Physical properties 2. Chemical properties, and 3. Interaction with a specific column filling (stationary phase). As the chemicals exit the end of the column, they are detected and identified electronically. The function of the stationary phase in the column is to separate different components, causing each one to exit the column at a different time (retention time). Other parameters that can be used to alter the order or time of retention are the carrier gas flow rate, and the temperature. Physical Components involve inlet port, Adsorption