methodsfordetectionofglycosylatedhemoglobin糖化血红蛋白的.pdf

Advances in Analytical Chemistry 分析化学进展, 2017, 7(3), 163-170 Published Online August 2017 in Hans. /journal/aac /10.12677/aac.2017.73022 Methods for Detection of Glycosylated Hemoglobin Zongli Huang, Zhirong Gan, Wan Lan, Jiating Hou, Xiaozhen Feng, Guocheng Han* College of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin Guangxi rd th st Received: Jul. 3 , 2017; accepted: Jul. 28 , 2017; published: Jul. 31 , 2017 Abstract Diabetes mellitus is one of the highest morbidity in the world. At present, the detection of glycosy- lated hemoglobin has become the standard diagnostic method of diabetes monitoring. Hemoglo- bin plays an important role in human body, as a macromolecular protein which is mainly respon- sible for carrying oxygen. And the combination of high blood glucose in patients with diabetes will induce glucose and hemoglobin in HbA1c generation function, blood oxygen capacity, so the de- termination of glycosylated hemoglobin in blood shows great significance. There are more than 30 methods used for the determination of glycosylated hemoglobin in clinical laboratories. According to the principle of the reaction, which can be divided into two categories, the first category is based on the different charge of GHb and non GHb, including ion exchange chromatography, electropho- resis, isoelectric focusing, etc. The second category is based on the structural characteristics of GHb, including affinity chromatography, ion capture, immunization, etc. This article overviewed detection methods of glycosylated hemoglobin in clinical practice. Keywords Diabetes Mellitus, Standard Diagnostic, Hemoglobin, Glycosylated Hemoglobin, Detection