溶菌酶（lysozyme）.doc

溶菌酶（lysozyme） Product description and instructions I. overview Dalian Luxue egg development Co. Ltd. (formerly Dalian biochemical products Co. Ltd.) is one of the largest egg powder processing enterprises, is currently Chinas largest manufacturer of egg white lysozyme. The company introduced INOVATECH Canada (CII, CLI) complete set of lysozyme production equipment and process, can produce hydrochloric acid egg white lysozyme (LYSOZYME CHLORIDE) 20 tons. Two, functional properties Egg white lysozyme E.C.3.2.1.7 is a glycoside hydrolase with a molecular measure of 14000-15000 and an isoelectric point of about 11. In order to lysozyme dissolve the cell wall of gram negative bacteria and bacteriolyze. The reason is that it can hydrolyze the -1.4 glycosidic bond between N- acetyl glucosamine and N- acetyl wall acid. This is the foundation of lysozyme application and the reason why it got its name. The optimum acidity is about PH8.0 in lysozyme lysis of the play, the best reaction temperature and the temperature of lysis is consistent with the requirements in the range of 50oC. Lysozyme has changed drastically in a wide range of PH1.2-11.3, and no change in lysozyme activity has been found. Therefore, lysozyme is insensitive to changes in pH. In the alkaline range, the stability is poor. The thermal denaturation of lysozyme is reversible, and its denaturation temperature is generally no higher than 70oC. In a word, lysozyme is quite stable. Three. The existence and vitality of lysozyme 1. lysozyme is widespread in nature Animal: egg white 3mg/ml human milk: 0.1-0.5mg/ml human tears: 7mg/ml human urine: 0.5-1.2g/ days Milk: 0.7mg/ gallon plants: papaya figs: 1.0-1.1mg\g insects: snails (snail enzymes) Microorganisms: many cells contain lysozyme. The 2. is the scope of their role of lysozyme, namely lysis spectrum. Lysozyme from different sources has different lysozyme. Only lysozyme lysis of gram positive bacteria clear egg. In these lysozyme, lysozyme activity was highest