生物化学笔记2（Biochemistry note 2）.doc

生物化学笔记2（Biochemistry note 2） Amino acids and proteins The classification of 20 amino acids that make up proteins 1 nonpolar amino acids They include glycine, alanine, valine, leucine, isoleucine, phenylalanine, and proline Polar amino acids 2 Polar neutral amino acids: tryptophan, tyrosine, serine, cysteine, methionine, asparagine, glutamine, threonine Acid amino acids: aspartic acid, glutamic acid Basic amino acids: lysine, arginine, histidine Of which: aromatic amino acids are: tryptophan, tyrosine, phenylalanine Amino acids belong to: proline Sulfur containing amino acids include cysteine and methionine Note: you can only remember the first word in memory, such as basic amino acids including: Lai Jing group Two, the physical and chemical properties of amino acids 1, dissociation and isoelectric point: The amino acid molecule has free amino and free carboxyl groups, and can combine with acids or bases to form salts. Therefore, it is a kind of amphoteric electrolyte. In a certain PH solution, the amino acids dissociate into positive ions and anions with the same degree of tendency and become facultative ions, which are electrically neutral, and at this point the PH of the solution is called the isoelectric point of the amino acid. 2. UV absorption properties of amino acids: Aromatic amino acids have the greatest UV at 280nm wavelength near the absorption peak, because most proteins containing these amino acids, amino acid residues and protein content is proportional to the wavelength of 280nm was measured by UV absorbance can be used for the quantitative analysis of protein solution. 3, three keto ketone reaction The amino groups of amino acids react with the three hydrate hydrate to form a blue violet compound. The maximum absorption peak of the compound is 570nm wave. Because the magnitude of the absorption peak is proportional to the amount of ammonia released by the amino acid, it can be used as a quantitative method for amino acid analysis. Three, peptide Two