细胞融合（cell fusion）.docVIP

细胞融合（cell fusion） cell fusion By culture and induction, the process of merging two or more cells into a single nucleus or multinucleated cell is called cell fusion (cell fusion) or cell hybridization (cell, hybridization). Cells of the same genotype fusion hybrid cells called homokaryons (homokaryon); from different genotypes of hybrid cells are called heterokaryons (heterokaryon). When the same kind of cells are cultured, the 2 cells that are joined together spontaneously merge, called spontaneous fusion, and the cells of different races must be treated by inducers to fuse, which is called induced fusion. There are three ways to induce cell fusion: biological methods (viruses), chemical methods (polyethylene glycol, PEG), physical methods (electric shock and laser). Some viruses such as Sendai virus, parainfluenza virus and Newcastle disease virus envelope fusion protein (fusion, protein) may be mediated by virus host cell fusion, fusion can also be mediated by cells and cells induced by the virus, so it can be used for UV inactivated cell fusion. Chemical and physical methods can result in changes in the molecular arrangement of membrane lipids. After removing the factors, the plasma membrane restores its original ordered structure and, in the course of its recovery, induces the fusion of cells in contact with each other. Cell fusion (cell, fusion), fusion of cells or protoplasts of two different genotypes under spontaneous conditions or artificial induction into a hybrid cell. The basic process includes cell fusion in heterokaryons (heterokaryon) formation of heterokaryons through mitosis in nuclear fusion, the formation of mononuclear hybrid cells. Normal fusion occurs when sexual reproduction occurs, as is the fusion of two gametes into one zygote. Human and rat cell fusion experiment is divided into three steps: firstly, the mouse antibody and green fluorescent antibody labeled with fluorescent dye, fluorescein (fluorescin) combined with Luo Danmings and red f