苯那普利对糖尿病大鼠肾皮质p21cip1蛋白表达的调节及其机制（Regulation of benazepril on the expression of p21cip1 protein in renal cortex of diabetic rats and its mechanism）.docVIP

下载本文档

3
0
约4.9千字
约 5页
2017-09-02 发布于河南
举报
版权申诉

苯那普利对糖尿病大鼠肾皮质p21cip1蛋白表达的调节及其机制（Regulation of benazepril on the expression of p21cip1 protein in renal cortex of diabetic rats and its mechanism）.doc

1、本文档共5页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

苯那普利对糖尿病大鼠肾皮质p21cip1蛋白表达的调节及其机制（Regulation of benazepril on the expression of p21cip1 protein in renal cortex of diabetic rats and its mechanism）

苯那普利对糖尿病大鼠肾皮质p21cip1蛋白表达的调节及其机制（Regulation of benazepril on the expression of p21cip1 protein in renal cortex of diabetic rats and its mechanism） Regulation of benazepril on the expression of p21CIP1 protein in renal cortex of diabetic rats and its mechanism 2011-05-09 Lin Shantan, Wu Yonggui, Yu Yi, Zhou Jianghua [Abstract] Objective To study the regulation and mechanism of benazepril on the expression of p21CIP1 protein in the renal cortex of diabetic rats. Methods rats were randomly divided into unilateral nephrectomy, control group, diabetes group and diabetes mellitus benazepril (10mg.kg-1., -1, gavage) treatment group. The expression of transforming growth factor beta 1 (TGF beta 1) mRNA in renal cortex was detected by Northern blot, and the expression of TGF, beta 1 and p21CIP1 protein in renal cortex was detected by Western blot. In addition, the activity of angiotensin converting enzyme (ACE) in the plasma, renal skin and medulla was determined by fluorescence spectrophotometry. Results after one week, the weight of the diabetic rats decreased obviously (P 0.05), and the kidney weight / body weight increased significantly (P 0.05). The activity of ACE in plasma decreased and the activity of ACE in the renal cortex increased. Northern blot showed that the diabetic group of renal cortex TGF beta 1 expression of mRNA than the control group increased by 63.6%. The expression of renal cortex TGF beta 1 and P21CIP1 protein also increased significantly; benazepril week on renal hypertrophy has an inhibitory effect on plasma and renal cortex and medulla ACE inhibitory activity were 89%, 70%, 70.5%, 47.7% respectively, expression suppression and renal cortex of TGF beta 1 and beta 1 TGF mRNA and p21CIP1 protein 49.5%, 60%. Conclusion benazepril can inhibit the high expression of p21CIP1 protein in the early stage of diabetic nephropathy, and its mechanism may be related to the high expression of TGF beta 1. Keywords benazepril; diabetic nephropathy; p21CIP1 protei