油松毛虫体内酶系对油松诱导抗性的响应机制-应用化学.PDF

第 50 卷 第 10 期 林 业 科 学 Vol. 50 ，No. 10 2 0 1 4 年 10 月 SCIENTIA SILVAE SINICAE Oct ． ，2 0 1 4 doi :10 ．11707 / j ．100 1-7488 ．20 14 1025 油松毛虫体 内酶 系对油松诱导抗性 的响应机 制* 张雄 帅 周 国娜 高宝嘉 (河 北 农业大学林学院 保定 07 1000 ) 关键词 : 油松 ; 油松 毛 虫 ; 诱 导抗性 ; 解 毒 酶 ; 保 护 酶 ; 消化 酶 中图分类号 : Q968 文献标识码 : A 文章编号 : 100 1 － 7488 (20 14 ) 10 － 0 18 1 － 07 Ｒesponse Mechanisms of Pine Caterpillar Enzymatic System to Pine Induced Ｒesistance Zhang Xiongshuai Zhou Guona Gao Baoj ia ( Forestry College ，Agricultural University of Hebei Baoding 07 1000 ) Abstract : To explore counter-defense response mechanism of pine caterpillar (Dendrolinus punctatus tabulaeformis Tsai et Liu ) to the induced insect-resistance of Chinese pine (Pinus tabulaef ormis ) ，the Chinese pine was fed by different amount pine caterpillars to obtain induced insect-resistance in Pingquan of Hebei Province ，and main detoxifying enzymes ， protective enzymes and digestive enzymes of pine caterpillars fed on the Chinese pine leaf were determined ． The results showed that most of enzymes in the pine caterpillars changed obviously after feeding various induced resistant pine leaves． Carboxylesterase ，glutathione-transferase ，superoxide dismutase ，peroxidase and protease activity of the lavae increased significantly with the increasing feeding stimulation ． Acetylcholinesterase ，lipase and amylase activity of the lavae had some change but no obviously linear variation ，and acetylcholinesterase activity had no change ． The results indicated that carboxyl esterase ，glutathione-s-transfe