稻瘟菌激发子CSBⅠ诱导水稻防御性相关酶的活性变化-作物学报.PDF

第 30 卷 第 6 期 作 　物 　学 　报 Vol. 30 , No. 6 2004 年 6 月 　613～617 页 ACTA AGRONOMICA SINICA pp. 613～617 　June , 2004 稻瘟菌激发子 CSB Ⅰ诱导水稻防御性相关酶的活性变化 李云锋　王振中 　贾显禄 (华南农业大学资源环境学院 ,广东广州 510642) ( ) 菌株 97151a 摘 　要 　选取抗稻瘟病水稻近等基因系 C101LAC 及背景品系 CO39 为材料 ,与稻瘟病菌 Magnaporthe grisea 分别组成高度非亲和性互作关系和亲和性互作关系。接种稻瘟菌细胞壁来源的糖蛋白激发子 CSB Ⅰ后,非亲和性互作 ( ) ( ) ( ) 品种叶片内过氧化物酶 POD 、苯丙氨酸解氨酶 PAL 和脂氧合酶 LOX 活性、绿原酸和木质素含量在互作早期已开始显 著增加 ,而亲和性互作品种则较晚才发生 ,其 POD 、PAL 和LOX 活性、绿原酸和木质素含量也远低于非亲和性互作品种。 绿原酸积累与 PAL 活性呈正相关 ;木质素积累与 POD 和 PAL 活性呈正相关。 关键词 　稻瘟菌 ;激发子 ;水稻 ;防御酶;抗性 中图分类号:S435 Activity Dynamics of Related Defense Enzymes Induced by an Elicitor CSB Ⅰfrom Magnaporthe grisea LI YunFeng , WANG ZhenZhong , J IA XianLu ( ) College of Resource and Environmental Sciences , South China Agricultural University , Guangzhou 510642 , Guangdong , China Abstract 　With two near isogenic lines of rice , studies were carried out for interaction between rice and a glycoprotein elicitor CSB Ⅰfrom Magnaporthe grisea . After treatment with the elicitor CSB Ⅰ, POD , PAL and LOX activity increased more remarkably and rapidly in the leaves of incompatible interaction rice than that of compatible interaction rice. The peak of PAL activity in incompatible interaction rice arrived at the time of 16 h earlier , and that of LOX activity at the time of 32 h earlier , than those in compatible interaction rice. The contents of c