ICAM-1、MCP-1表达水平及基因多态性及EV71手足口病.pdf

I CAIII卜1、NOP-1表达水平及基因多态性与Ev71手足口病 中文摘要 易感因素。 方法收集手足13病患儿急性期(发病24小时内)大便标本，常规处理大便标本，运 进行直线相关分析。运用限制性片段长度多态性一聚合酶链反应(PCR．RFLP)检测 况，并取样采用碱基直接测序进行验证，对数据进行统计学分析。 ICAM．1K469E位点基因型分布存在显著差异(X 组A等位基因频率明显低于对照组(X MCP．1基因型分布、等位基因频率与对照组比较均无统计学意义(P0．05)。 手足口病发生率。③MCP．1基因A．2518G位点基因多态性与EV71手足口病无关。 硕士研究生：胡静飞01,科学) 指导老师：陈宗波 关键词：肠道病毒7l型，手足口病，细胞间粘附分子1，单核细胞趋化 因子1，基因多态性 leveland The betweenICAM—l，MCP-1 relationship plasma gene in withthe ofenterovirus71infection polymorphismssusceptibility andMouthDisease Hand，Foot Abstract To the betweenICAM-1，MCP-1leveland objective plasma gene studyrelationship with ofenterovirus71 infectionin and the Hand，Foot polymorphismssusceptibility Mouth ． Disease． Methodsreverse chain madeuseof reaetion(RT-PCR)．we By transeriptionpolymerase in and EV71 todetectenterovirus71 theserumof Mouth specificprimer Hand,Foot children．PCR_RFLP anddirect were Disease(HFMD) techniques sequencing todeterminethe of1CAM-1K469EandMCP-1A一2518G in performed genotypes gene andMCP-1 EV7linfectionandinnorm