新生猪胰岛保存运输的试验研究.pdfVIP

16 卷 3 期 生命科学研究 灾燥造援员6 晕燥援3 圆园12 年6 月 蕴蚤枣藻 杂糟蚤藻灶糟藻 砸藻泽藻葬则糟澡 Jun. 圆园12 新生猪胰岛保存运输的 验研究 叶 斌, 董 琼, 马小倩, 聂 唯 （中南大学 湘雅三医院, 中国湖南 长沙4100 13） 摘 要: 探索 生猪胰岛较长时间有效保存运输的方法, 使不同胰岛应用单位间分享胰岛资源成为可能. 用胶 原酶消化 分离得到 生猪胰岛, 培养5 d 后收集, 将所得胰岛置于自行设计制作的保存运输系统内, 分别于 置入后2 4 5 7 h, 分次取出胰岛进行双硫腙渊DTZ 冤染色 吖啶橙荧光染色 体外葡萄糖刺激胰岛 分泌实验, 确定胰岛细胞的功能和活性, 并使用流式细胞仪检测凋亡情况. 使用本研究的保存运输方法, 在5 h 以内, 胰 岛的功能和活性无明显受损, 超过5 h, 胰岛明显凋亡, 功能 活性显著降低. 在脱离培养箱环境情况下, 本方法 5 h 内可以有效保存胰岛. 关键词: 胰岛曰 功能曰 活性曰 保存曰 运输 中图分类号: R322. 5+7 文献标识码: A 文章编号: 1007鄄7847(2012)03鄄0237鄄05 The Experimental Study on Preservation and Transportation of Newborn Porcine Islets YE Bin, DONG Qiong, MA Xiao鄄qian, NIE Wei (The Third X iangya Hosp ital of Central South Unive rs ity, Changsha 4 10013, H unan, China) Abstract: In order to investigate an effective method for long鄄time transportation of newborn porcine islets (NPI), after 5 days of culture, the NPI were stored in transportation system which was designed and manu鄄 factured by us to keep the NPI survival. DTZ （Dithizone ）staining, acridine orange staining and in v itro glu鄄 cose stimulation experiment were done after 2, 4, 5, and 7 hours. The apoptosis was detected by flow cytometry. With the transportation system, the function and activity of NPI did not change significantly within 5 hours. However after 5 hours, the increased apoptosis and decreased function and activity of the NPI were markedly noticed. The storage and transportation system can store NPI effectively within 5 hours without incubator. Key words: islet ；function ；activity ；preservation ；transportation