SrDNA序列关键位点的信息论分析-食品与生物技术学报.PDFVIP

第29 卷第5 期 食 品 与生 物 技 术学 报 Vol. 29 No.5 2010 年9 月 Journal of Food Science and Biotechnology Sept 2010 : 2010 16S rDNA 序列关键位点的信息论分析 1,2 2 2 * 1,2 周大为 , 于雪飞 , 周少刚 , 李炜疆 ( 1. 江南大学 工业生物技术教育部重点实验室, 江苏无锡214122; 2. 江南大学 生物工程学院, 江苏无锡214122) : 16S rDNA 中含有较多的菌种特异性 息,但是如何使用这些 息进行菌种鉴定却因不 同菌种间序列的特征变化缺少规律而变得困难作者使用 息论方法对那些种内较保守,种间变 异明显的关键位点进行研究结果表明, 16S rDNA 序列中仅有少数位点对菌种的分类是重要的 基于少数关键位点的相似度比传统的全序列鉴定有着更好的统计学特性 : 16S rDNA; 分类; 信 论; 熵 :Q 93.331 : A InformationTheory Analysis for Key Sites of 16S rDNA Sequence 1,2 2 2 * 1,2 ZHOU Dawei , YU Xuefei , ZHOU Shaogang , LI Weijiang (1. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangan University, Wuxi 214122,China; 2 School of Biotechnology, Jiangnan University, Wuxi 214122, China) Abstract 16S rDNAs carry significant speciesspecific genetic information, but species identification is not straight forward because the sequence identity varies greatly for different species In this manuscript, we analyze the key sites that are well conserved within species but significantly varied between species using an information theoretic approach. The method proposed by uswasjust using the sites (key sites) which contained moregenetic informationthan that of the normal sites of the sequences, to classify the bacterium rather than using the overall sequence. Only afew sites of thewhole sequences are responsiblefor theclassification of species. The results presented here demonstrated that keysites based similarity measures have much better statistical characteristics than that of the tr