端大蓟马取食对植物防御酶活性及丙二醛含量的影响.PDFVIP

安徽农业大学学报, 2017, 44(4): 716-719 Journal of Anhui Agricultural University [DOI] 10.13610/ki.1672-352x016 网络出版时间：2017/8/11 12:00:02 [URL] /kcms/detail/34.1162.S1200.032.html 端大蓟马取食对豆科植物防御酶活性及丙二醛含量的影响 沈登荣，何 超，徐慧琼，雷映红，朱敏瑜，田学军，袁盛勇 (红河学院生命科学与技术学院，蒙自 661199) 摘 要：以3 种豆科植物（菜豆、豇豆和蚕豆）为植物材料，研究端大蓟马成虫和若虫取食胁迫3 种豆科植物 后，其叶片超氧化物歧化酶（SOD）、过氧化物酶（POD ）、过氧化氢酶（CAT ）活性和丙二醛（MDA ）含量的变 化。结果表明，随着取食时间的延长，3 种酶活性和MDA 含量逐渐升高。若虫和成虫取食胁迫可引起3 种酶活性 和MDA 含量上升，其中若虫取食胁迫下的3 种酶活性和MDA 含量最高。端大蓟马的取食胁迫可引起3 种豆科植 物POD 、CAT 活性和MDA 含量的显著增加（P ＜0.05）。表明豆科植物SOD、POD 、CAT 和MDA 变化与植物种 类、端大蓟马虫态和取食时间有关，豆科植物的抗性与体内的POD 、CAT 和MDA 有密切联系。 关键词：豆科植物；端大蓟马；取食胁迫 中图分类号：S433.1 文献标识码：A 文章编号：1672352X (2017)04071604 Effects of Megalurothrips distalis feeding stress on the activities of defense enzymes and MDA content in leguminous plants SHEN Dengrong, HE Chao, XU Huiqiong, LEI Yinghong, ZHU Minyu, TIAN Xuejun, YUAN Shengyong (College of Life Science and Technology, Honghe University, Mengzi 661199) Abstract: The activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and malondial- dehyde (MDA) content were evaluated in the leaves of three leguminous plants (Phaseolus vulgaris, Vigna un- guiculata and Vicia faba) by feeding stress of Megalurothrips distalis . The results showed that with the extension of feeding time, the activities of SOD, POD, CAT and MDA content increased. The activities of the enzymes and MDA content increased by feeding stress of both nymph and adult and reached the highest by feeding stress of nymph. Feeding stress of M. distalis could lead to a significant increase of the activities of POD, CAT and MDA content in three leguminous plants (P0.05). The results indicated that the changes of SOD, POD, CAT and MDA in leguminous plants are associated with plant species, the