螺旋藻培养条件响应面法优化的研究.pdfVIP

※工艺技术 食品科学 2007, Vol. 28, No. 08 147 谯顺彬1,2,3，张奕婷1,2，迟海洋1,2，张义明1,3,* (1.贵州省发酵工程与生物制药重点实验室，贵州 贵阳 550003； 2.贵州大学化学工程学院，贵州 贵阳 550003；3.贵州科技工程职业学院，贵州 贵阳 550008) ：本研究设计了用于螺旋藻培养的新型气升式光生物反应器，并用响应曲面法对其培养条件进行优化。试 验选取影响螺旋藻生长的四个关键因素即光照强度、通气量、培养时间和接种量，并对其最佳水平范围进行研究， 建立了以藻体干重为响应值的二次多项式方程。试验结果表明，四个因素对藻体生长的影响大小依次为光照强度、 培养时间、装液量、通气量；对方程解逆矩阵可知，当光照强度、通气量、培养时间和接种量分别达最佳水平 4400lx、212.2L/h、8.8d和7.2L时，DW 最大值为1.277g/L。 ：气升式光生物反应器；螺旋藻；响应曲面法 Optimization of Cultivation Conditions for Biomass by Spirulina. platensis Using RSM 1,2,3 1,2 1,2 1,3, QIAO Shun-bin ，ZHANG Yi-ting ，CHI Hai-yang ，ZHANG Yi-ming * (1.Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guiyang 550003, China； 2.College of Chemical Engineering, Guizhou University, Guiyang 550003, China； 3.Guizhou Technological Engineering and Vocational College, Guiyang 550008, China) Abstract ：In this paper, a novel air-lift photo-bioreactor was designed to cultivate the Spirulina platensis, employing the response surface methodology to optimize the culture conditions of biomass concentration. Four critical factors selected for investigation were light intensity, air flow，time of cultivation and volume of medium. Then, research the optimization concentration levels and the relationship between these factors and building a quadratic regression equation with DW as the dependent. According to the experiment results, the ordinal relation from strong to inferior influence the growth of SP is light intensity, time of cu