艾纳香油对晒伤小鼠皮肤氧化应激及DNA损伤影响.docVIP

艾纳香油对晒伤小鼠皮肤氧化应激及DNA损伤影响 　摘 要 观察艾纳香油对UVB照射后Blab/C小鼠晒伤皮肤氧化应激及DNA损伤的影响。通过建立4组小鼠晒伤模型，在5个不同时间相点测定小鼠皮肤晒伤组织厚度和含水量，利用可见光分光光度法测各组皮肤组织中超氧化物岐化酶（SOD）、丙二醛（MDA）、还原型谷胱甘肽（GSH）和8-羟基脱氧鸟苷（8-OHdG）的含量。结果表明：艾纳香油可明显加快晒伤创面处结痂脱落，抑制晒伤皮肤组织增厚（Plt;0.05），减少晒伤组织皮肤含水量的丧失（Plt;0.05，Plt;0.01）；在整个治疗过程，能明显提高小鼠血清SOD活性（Plt;0.05，Plt;0.01），降低皮肤MDA含量（Plt;0.05），升高皮肤GSH的水平（Plt;0.05），降低皮肤8-OHdG含量（Plt;0.01）。艾纳香油可抵抗UVB晒伤后小鼠表皮增厚，减少光产物表达，并通过清除氧自由基，提高抗氧化酶活性而发挥抗氧化作用。　　关键词 艾纳香油 ；中波紫外线 ；抗氧化　　分类号 R284.1 ；S567.23+9 Doi：10.12008/j.issn.1009-2196.2016.02.012　　Effects of Blumea Balsamifera Oil on Oxidative Stree　　and DNA Damages in a Model of Skin Sunburn in Mice　　LI Xiaoting1，2，3） WANG Dan2，3） PANG Yuxin22，3）　　YANG Quan1） FAN Zuowang1，2，3） MA Qingsong1，2，3） XU Luofeng1，2，3）　　（1 School of Traditional Chinese Medicine， Guangdong Pharmaceutical University，　　Guangzhou， Guangdong 510006， China；　　2 Tropical crops Genetic Resources Institute， CATAS， Danzhou， Hainan 571737， China；　　3 Hainan Provincial Engineering Research Center for Blumea Balsamifera，　　Danzhou， Hainan 571737， China）　　Abstract To evaluate the protective effect of Blumea balsamifera oil on UVB irradiation-induced skin sunburn in Blab/C mice. The sunburn mouse models were established by UVB irradiation. The mice were randomly divided into four groups. The skin erythema and edema were assessed. The thickness of epidermis and the water content of tissues were determined and the levels of superoxide dismutase （SOD）， malondialdehyde （MDA），glutathione （GSH）， 8-Hydroxy-desoxyguanosine in its epidermis were measured with visible spectrophotometry methods at 5 different time points. Results： The skin loss of Blumea balsamifera oil group was accelerated. The topical application of Blumea balsamifera oil resulted in a significant decreasing in UVB-induced increases in skin thickness. Further more， Blumea balsamifera oil treatments also resulted in a significant increasing the level of SOD and GSH， decreasing the loss of skin water content and MDA， especially depressing in UV