ph型有机磷水解酶生物传感器的稳态模型 steady-state model of ph-based organophosphorus hydrolase biosensor.pdfVIP
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ph型有机磷水解酶生物传感器的稳态模型 steady-state model of ph-based organophosphorus hydrolase biosensor
第21卷第12期 传感技术学报 V01.21No.12
CHINESE OFSENSORSAND
2008年12月 JOURNAL ACTUATORS Dec.2008
Modelof Biosensor’
Steady-StatepH—BasedOrganophosphorusHydrolase
WANG Weil,YAO Zhen-xiul
Li-hong“,WANGLian92,SHENYu—lon91,YANG
Hebei
Teachers’College,Tangshan063000,China;
,1.DepartmentofChemistry,Tangshan 、
andEnvironmental
L2.CollegeofChemistryScience,HebeiUniversity,BaodingHebei071002,China,
Abstract:Onthebaseofthe ofbiosensors
workingprinciple immobilizingorganophosphorushydrolase,a
mathematicmodelofbiosensorsimmobilizing and electrodeasthetrans-
organophosphorushydrolasepH
ducersiSsetuD.Themodelcombinesthe ofdiffusionand reactioninthemembrane.
processes enzymatic
Solutionofthedifferential thedetection ofthebiosensorssummarizesthe
equationsdescribing process
mainfactors the characteristicsofthe factors the
influencingresponse biosensors.Keyinfluencingsystem
as reaction bufferconcentrationarediscussedin
response,suchenzyme order,Thielemodulus,and detail.
Itis toobtaina linear by an electrodewitha Thiele
possible good responsedesigningenzyme highenough
the
modulusand addedbufferconcentration.Itisfoundthatthemodelisin
byappropriatelycontrolling
reasonable withthe results.
agreementexperiment
of detection
Keywords:organophosphor
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