4种不同结核分枝杆菌检测方法对结核病的诊断价值比较_张立群.pdfVIP

2010 20 11 ·1633 · ·　 · 4 张立群, 王云霞, 周　敏, 黄君富, 府伟灵 (, 400038) :　、、 , 。　、 、 172 ;、 。　、、 36.0%、50.6%、11.6%75.6%;4 81.6%、73.7%、26.3% 88.2%; 100.0%、67.7%、100.0%34.4%。　、, 、 、, 。 :;;; :R521　　:A 　　:1005-4529(20 10)11-1633-03 Diagnosis of Mycobacterium tuberculosis: A Clinical Evaluation of Four Detection Methods ZHANG Li-qun, WANG Yun-xia, ZHOU M in, H UANG Jun-fu, FU W i-ling (The F irs t A f f i li ated H osp i ta l, th e Thir d M i li tary Med i ca l Un iv ersity , Chong qing 400038, China ) Abstract:OBJECTIVE T o compar th g n chip t chnology (GCT )with th dot immunogold chromatographic assay(DICA), th acid-fast stain sm ar m thod and th purifi d prot in d rivativ tub rculin skin t sting (PP D) for d t ction of My cobacter ium tubercu los is in ord r to valuat th clinical application valu of diff r nt m thods. MET ODSS v nty-six tub rculosis cas s, and 96 nontub rculosis cas s w r d t ct d by th four m thods. RESULTSTh positiv rat of th g n chip, DICA, A FS and PP D w r 36.0%, 54.0%, 50.6%, 11.6%and 75.6%, r sp ctiv ly .Th s nsitivity of th four m thods w r 81.6%, 73.7%, 26.3% and 88.2%, w hil th sp cificity of th four m thods w r 100.0%, 67.7%, 100.0% and 34.4%, r sp ctiv ly.CONCLUSIONS Compar d w ith DICA, A FS and PPD, th T B g n chip is simpl , rapid, s nsitiv and sp cific for d t cting tub rculosis, w hich can b tak n as a h lpful m thod for arly and rapid d t ction of M .tubercu los is . Key words:G n chip;tub rculosis;My cobacter ium tubercu losis, d t ction m thods 　　 (DICA )、 ,, (AFS) , (PPD), , , 。