a high-precision micropipette sensor for cellular-level real-time thermal characterization再一个高精度微量吸液管传感器实时热特性.pdfVIP

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a high-precision micropipette sensor for cellular-level real-time thermal characterization再一个高精度微量吸液管传感器实时热特性.pdf

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a high-precision micropipette sensor for cellular-level real-time thermal characterization再一个高精度微量吸液管传感器实时热特性

Sensors 2011, 11, 8826-8835; doi:10.3390/s110908826 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article A High-Precision Micropipette Sensor for Cellular-Level Real-Time Thermal Characterization Ramesh Shrestha 1, Tae-Youl Choi 1,*, Wonseok Chang 2 and Donsik Kim 3 1 Department of Mechanical and Energy Engineering, University of North Texas, 3940 N. Elm St, Denton, TX 76207, USA; E-Mail: E-Mail: rameshstha@ 2 Department of Nanomechanical Systems, Korea Institute of Machinery and Materials, 156 Gajungbukno, Yuseong-gu, Daejeon 305-343, Korea; E-Mail: paul@kimm.re.kr 3 Department of Mechanical Engineering, POSTECH, San 31 Hyoja-Dong, Nam-Gu, Pohang, Gyungbuk 790-784, Korea; E-Mail: dskim87@postech.ac.kr * Author to whom correspondence should be addressed; E-Mail: choi@; Tel.: +1-940-565-2198; Fax: +1-940-369-8675. Received: 5 August 2011; in revised form: 7 September 2011 / Accepted: 7 September 2011 / Published: 13 September 2011 Abstract: We report herein development of a novel glass micropipette thermal sensor fabricated in a cost-effective manner, which is capable of measuring steady thermal fluctuation at spatial resolution of ~2 µm with an accuracy of ±0.01 °C. We produced and tested various micrometer-sized sensors, ranging from 2 µm to 30 µm. The sensor comprises unleaded low-melting-point solder alloy (Sn-based) as a core metal inside a pulled borosilicate glass pipette and a thin film of nickel coating out