a microarray approach to identify genes involved in seed-pericarp cross-talk and development in peach微阵列的方法来识别基因参与seed-pericarp相声和桃子的发展.pdfVIP

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a microarray approach to identify genes involved in seed-pericarp cross-talk and development in peach微阵列的方法来识别基因参与seed-pericarp相声和桃子的发展.pdf

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a microarray approach to identify genes involved in seed-pericarp cross-talk and development in peach微阵列的方法来识别基因参与seed-pericarp相声和桃子的发展

Bonghi et al. BMC Plant Biology 2011, 11:107 /1471-2229/11/107 RESEARCH ARTICLE Open Access A microarray approach to identify genes involved in seed-pericarp cross-talk and development in peach 1† 2† 1 2 1 1 Claudio Bonghi , Livio Trainotti , Alessandro Botton , Alice Tadiello , Angela Rasori , Fiorenza Ziliotto , Valerio Zaffalon 1, Giorgio Casadoro2 and Angelo Ramina1* Abstract Background: Field observations and a few physiological studies have demonstrated that peach embryogenesis and fruit development are tightly coupled. In fact, attempts to stimulate parthenocarpic fruit development by means of external tools have failed. Moreover, physiological disturbances during early embryo development lead to seed abortion and fruitlet abscission. Later in embryo development, the interactions between seed and fruit development become less strict. As there is limited genetic and molecular information about seed-pericarp cross- talk and development in peach, a massive gene approach based on the use of the μPEACH 1.0 array platform and quantitative real time RT-PCR (qRT-PCR) was used to study this process. Results: A comparative analysis of the transcription profiles conducted in seed and mesocarp (cv Fantasia) throughout different developmental stages (S1, S2, S3 and S4) evidenced that 455 genes are differentially expressed in seed and fruit. Among differentially expressed genes some were validated as markers in two subsequent years and in three different genotypes. Seed markers were a LTP1 (lipid transfer protein), a PR (pathogenesis-related) protein, a prunin and LEA (Late Embryogenesis Abundant) protein, for S1, S2, S3 and S4, respectively. Mesocarp markers were a RD22-like protein, a serin-ca