a novel “reactomics” approach for cancer diagnostics一种新型u201creactomicsu201d为癌症诊断方法.pdfVIP

Sensors 2012, 12, 5572-5585; doi:10.3390/s120505572 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article A Novel “Reactomics” Approach for Cancer Diagnostics Sofiya Kolusheva 1,†, Rami Yossef 2,†, Aleksandra Kugel 2, Nirit Hanin-Avraham 1, Meital Cohen 2, Eitan Rubin 2 and Angel Porgador 2,* 1 Ilse Katz Institute for Nanoscale Science and Technology, Ben Gurion University of the Negev, Beer Sheva 84105, Israel; E-Mails: kolushev@bgu.ac.il (S.K.); nirithanin@ (N.H.-A.) 2 The Shraga Segal Department of Microbiology and Immunology and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva 84105, Israel; E-Mails: yossefra@bgu.ac.il (R.Y.); sasha.kugel@ (A.K.); kupervaser@ (M.C.); erubin@bgu.ac.il (E.R.) † These authors contributed equally to this work. * Author to whom correspondence should be addressed; E-Mail: angel@bgu.ac.il; Tel.: +972-8-647-7283; Fax: +972-8-647-7626. Received: 3 February 2012; in revised form: 30 March 2012 / Accepted: 23 April 2012 / Published: 2 May 2012 Abstract: Non-invasive detection and monitoring of lethal diseases, such as cancer, are considered as effective factors in treatment and survival. We describe a new disease diagnostic approach, denoted “reactomics”, based upon reactions between blood sera and an array of vesicles comprising different lipids and polydiacetylene (PDA), a chromatic polymer. We show that reactions between sera and such a lipid/PDA vesicle array produce