中国农科院历年考博试题（China Kaobo questions over the years.）.docVIP

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中国农科院历年考博试题（China Kaobo questions over the years.）.doc

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中国农科院历年考博试题（China Kaobo questions over the years.）

中国农科院历年考博试题（China Kaobo questions over the years.） (8. 3) Chinese Kaobo questions over the years. Assumes. Ferry (gold +6): Thanks (8. 3) Chinese Kaobo questions over the years. China Kaobo questions over the years. 2002 China academy doctoral introduction to gene engineering test A short answer, What is the 1, DNA electrophoresis in agarose polyacrylamide, the difference? 2, gives two kinds of methods of transgenic animal, and explain its principle. Principle 3, dideoxy sequencing method. 4, in Arabidopsis or maize as an example, explains the transposon tagging method of gene transfer principle. The principle and application of 5, Southern blot. Three, try to discuss progress of plant genetic engineering research and the significance in agricultural production. 2001 China academy doctoral introduction to gene engineering test A glossary The 1 restriction endonuclease isoschizomers 2, 3, 4, 2 and 5 ring ribozyme, hat 6, Ti 7, T-DNA 8, MB plasmid tRNA 9, antisense tRNA 10, antisense strand. 11, alpha 12, complementary gene library 13, cDNA 14, chromosome walking Two short answer questions. 1, two kinds of methods for plant gene transfer and discusses its principle?. The basic principle, 2 Southern blot, this method has any application. 3, phage and COS as the carrier what is the difference? The principle and application of 4, AFLP 5, what is the difference between the ordinary PCR and RAPD, what is PCR? 6, what is the binary vector, the assembly process and its mechanism of action? Three, to determine the problem 1, no matter what kind of methods are available for PBR322 vector transformation 2, enter the foreign DNA is bacterial degradation, because bacteria only modified their DNA, no modification of foreign DNA 3, only the end can be connected with clay For primer synthesis cDNA chain 4, with its own cDNA, often is not complete 1998 China academy doctoral introduction to gene engineering test What is a genetic engineering, genetic engineering has significance in a