原创力文档an automated cell-counting algorithm for fluorescently-stained cells in migration assays一个自动细胞计数算法fluorescently-stained细胞迁移化验.pdfVIP
- 1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
- 2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
an automated cell-counting algorithm for fluorescently-stained cells in migration assays一个自动细胞计数算法fluorescently-stained细胞迁移化验
Biological Procedures
Online
An automated cell-counting algorithm for
fluorescently-stained cells in migration assays
Al-Khazraji et al.
Al-Khazraji et al. Biological Procedures Online 2011, 13:9
/content/13/1/9 (19 October 2011)
Al-Khazraji et al. Biological Procedures Online 2011, 13:9
/content/13/1/9 Biological Procedures
Online
METHODOLOGY Open Access
An automated cell-counting algorithm for
fluorescently-stained cells in migration assays
1 1 1 1,2*
Baraa K Al-Khazraji , Philip J Medeiros , Nicole M Novielli and Dwayne N Jackson
Abstract
A cell-counting algorithm, developed in Matlab®, was created to efficiently count migrated fluorescently-stained
cells on membranes from migration assays. At each concentration of cells used (10,000, and 100,000 cells), images
were acquired at 2.5 ×, 5 ×, and 10 × objective magnifications. Automated cell counts strongly correlated to
manual counts (r2 = 0.99, P 0.0001 for a total of 47 images), with no difference in the measurements between
methods under all conditions. We conclude that our automated method is accurate, more efficient, and void of
variability and potential observer bias normally associated with manual counting.
Keywords: automated cell counting, threshold, migration assays, manual cell counting
Background absorbance microplate readers [10-12]. These microplate
Traditionally, in vitro cell-counting methodologies consist
您可能关注的文档
- allozyme variation in fourteen natural populations of drosophila melanogaster collected from different regions of france黑腹果蝇的异型酶14个自然种群的变化收集了来自不同地区的法国.pdf
- allozyme frequency changes in two inverse sequences of environments in drosophila melanogaster异型酶两个逆序列频率变化环境的黑腹果蝇.pdf
- allpaths 2 small genomes assembled accurately and with high continuity from short paired readsallpaths 2小基因组组装准确、高连续性短配对阅读.pdf
- all-plastic electrochemical transistor for glucose sensing using a ferrocene mediator所有的塑胶电化学葡萄糖感应使用二茂铁中介的晶体管.pdf
- allozyme variation in natural populations of lymantria dispar (lepidoptera)异型酶变化的自然种群lymantria dispar(鳞翅目).pdf
- alpha7 nicotinic acetylcholine receptor is a target in pharmacology and toxicologyalpha7烟碱乙酰胆碱受体在药理学和毒理学的目标.pdf
- allylation of functionalized aldehydes by potassium allyltrifluoroborate catalyzed by 18-crown-6 in aqueous media功能化醛的烯丙基化钾allyltrifluoroborate 18-crown-6催化的水媒体.pdf
- all-solid-state textile batteries made from nano-emulsion conducting polymer inks for wearable electronics全固态电池纺织制成nano-emulsion导电聚合物油墨可穿戴电子产品.pdf
- alpha-actinin-binding antibodies in relation to systemic lupus erythematosus and lupus nephritisalpha-actinin-binding抗体在系统性红斑狼疮、狼疮肾炎.pdf
- alpha 1-antitrypsin retention in an ectopic liverα1-antitrypsin保留在一个异位肝.pdf
- an automated and simple method for brain mr image extraction一个自动化的大脑图像提取先生和简单的方法.pdf
- an automatic instrument to study the spatial scaling behavior of emissivity一个自动仪器研究发射率的空间扩展行为.pdf
- an automated stochastic approach to the identification of the protein specificity determinants and functional subfamilies自动随机方法识别蛋白质的特异性决定因素和功能亚科.pdf
- an automatic system for continuous monitoring of co2, h2s, so2 and meteorological parameters in the atmosphere of volcanic areas自动连续监测系统的二氧化碳、硫化氢、二氧化硫和气象参数在火山地区的大气.pdf
- an autosomal trisomy in cattle牛的一个常染色体三染色体细胞.pdf
- an ecological quantification of the relationships between water, sanitation and infant, child, and maternal mortality生态之间的关系量化的水、卫生设施和婴儿,儿童和孕产妇死亡率.pdf
- an early fire detection algorithm using ip cameras早期火灾探测算法使用ip摄像机.pdf
- an ecological and conservation perspective on advances in the applied virology of zoonoses生态保护的角度上提出应用病毒学的人畜共患病.pdf
- an attempt to modify allelic frequencies at the adh locus of a drosophila melanogaster population in a tropical environment试图修改adh位点的等位基因频率在热带环境中黑腹果蝇的人口.pdf
- an economical tandem multiplex real-time pcr technique for the detection of a comprehensive range of respiratory pathogens一种经济同步多路实时pcr技术,全方位的呼吸道病原体的检测.pdf
文档评论（0）