中药化学实用技术（Practical technique of Chinese Medicine Chemistry）.doc

中药化学实用技术（Practical technique of Chinese Medicine Chemistry） Practical technology of Chinese Medicine Chemistry The | application materials | courseware and lesson plans | study guide | | teaching video simulation training exercises | | case examination | evidence materials | interactive teaching A training project Two training project Three training project Four training project Five training project Six training project Seven training project Eight training project Nine training project Ten training project Eleven training project Does training guide Chinese chemical training practical technical guidance Isolation and identification of free anthraquinones in rhubarb five training project [Objective] training The 1. is capable of using reflux extraction method to extract total anthraquinones in Rhubarb 2. proficient in pH gradient extraction of free anthraquinones in rhubarb were isolated. 3. correct judgment of color reaction and TLC results [training] principle Rhubarb contains emodin anthraquinone glycosides, the use of training of anthraquinones in Rhubarb can be soluble in ethanol and extraction, according to the solubility of free anthraquinones and anthraquinone glycosides in water and ether in different extraction separation method. The separation of free anthraquinone is the use of anthraquinones in different acidic, using pH gradient extraction. [materials] training Round bottom flask condensing tube mortar bath separatory funnel beaker flask dish tube chromatography cylinder pH dipstick TLC silica gel CMC-Na rhubarb powder 95% 5%KOH 5%Na2CO3 5%NaHCO3 chloroform ether and ethanol hydrochloride 0.5%NaOH Xinhua filter paper chromatography (20cm * 7cm) - benzene ethyl acetate (8: 2) benzene methanol (8: 1) toluene ammonia 0.5% magnesium acetate 1% Rhein chloroform solution 1% emodin solution 1% aloe emodin chloroform solution [training] steps Total 1. ethanol extract preparation Extraction of anthraquinones Take crude rhubarb powder 50g, 500ml in the round bo