critical appraisal of the mtt assay in the presence of rottlerin and uncouplers关键mtt试验鉴定rottlerin和解偶联剂的存在.pdfVIP

Critical Appraisal of the MTT Assay in the Presence of Rottlerin and Uncouplers Emanuela Maioli, Claudia Torricelli, Vittoria Fortino, Filippo Carlucci, Valentina Tommassini, and Adriana Pacini Abstract Rottlerin is a natural product isolated from Mallotus philippinensis. This polyphenolic compound, origi- nally described as a selective inhibitor of PKCδ, can inhibit many other PKC-unrelated kinases and has a number of biological actions, including mitochondrial uncoupling effects. We recently found that Rot- tlerin inhibits the transcription factor nuclear factor κB in different cell