氯化汞引起的家兔急性肾功能不全.docVIP

氯化汞引起的家兔急性肾功能不全 摘要 【目的】学习用氯化高汞复制急性中毒性肾功能不全的动物模型；观察急性肾功能不全时尿蛋白、血肌酐、尿肌酐、肾酚红排泄率的变化及肾形态学的改变，并根据实验结果分析和讨论致病因素及导致急性肾功能不全的可能发病机制。【方法】采用苦味酸沉淀蛋白法对正常家兔和急性肾功能不全的家兔血肌酐、尿肌酐、酚红排泄率进行测定，对数据进行统计学的处理，肉眼肾形态学的改变。【结果】与正常组的家兔对比，实验组急性肾功能不全的家兔的血清肌酐升高，尿肌酐（P0.05）明显降低，每分钟的尿量（P0.01）减少，内生肌酐清除率显著下降，酚红排泄率下降，肾系数略有增大。【结论】重金属等毒物可直接损害肾小管，引起肾脏急性中毒性肾功能不全。急性肾功能不全会引起家兔肾小球滤过率下降，引起肾小管上皮细胞变性、坏死，引起肾血管收缩，肾血流减少，肾灌注压下降，肾小管排泌功能、肾小管上皮细胞重吸收功能障碍。 关键词： 氯化汞；肾功能不全；血清肌酐；尿肌酐 Abstract:【Objective】learn to use chlorinated high mercury copy acute toxic renal insufficiency of animal model. Acute renal not observe full-time urine protein, creatinine, serum creatinine, renal phenol red urine discharge rate changes and renal morphology change, and according to the analysis of experimental results and discussion of pathogenic factors and results in acute renal insufficiency may pathogenesis.【Method】the GuWeiSuan protein deposition method to study and normal acute renal all serum creatinine, urine rabbit serum creatinine, phenol red excretion rate for the determination of the statistical data, the processing, the naked eye kidney the morphology of the change. 【Aesult】the normal group and the rabbit, and the contrast of acute renal insufficiency rabbit serum creatinine, urine creatinine increased (P 0.05) reduced significantly, and each minute of urine output (P 0.01), reduce the endogenous creatinine clearance decreased significantly, phenol red excretion rate, renal coefficient increases slightly.【Conclusion】other kinds of heavy metals can be directly damage caused renal tubular, acute toxic renal insufficiency. Not all the cause acute renal rabbit glomerular filtration rate drop, cause renal tubular epithelial cells degeneration and necrosis, cause renal vascular contraction, renal decreased blood flow, renal perfusion pressure drop, renal tubular row secrete function, renal tubular epithelial cells heavy absorption function obstacle. Keyword: Chlorinated mercury; Renal insufficiency; Serum creatinine; Urine creatinine