原创力文档a mitochondria-dependent pathway mediates the apoptosis of gse-induced yeastmitochondria-dependent通路介导的细胞凋亡gse-induced酵母.pdfVIP
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a mitochondria-dependent pathway mediates the apoptosis of gse-induced yeastmitochondria-dependent通路介导的细胞凋亡gse-induced酵母
A Mitochondria-Dependent Pathway Mediates the
Apoptosis of GSE-Induced Yeast
1 1,2 2 1 1,2 1 1,2
Sishuo Cao , Wentao Xu *, Nan Zhang , Yan Wang , YunBo Luo , Xiaoyun He , Kunlun Huang *
1 Laboratory of food safety and molecular biology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, People’s Republic of China,
2 The Supervision, Inspection and Testing Center of Genetically Modified Organisms, Ministry of Agriculture, Beijing, People’s Republic of China
Abstract
Grapefruit seed extract (GSE), which has powerful anti-fungal activity, can induce apoptosis in S. cerevisiae. The yeast cells
underwent apoptosis as determined by testing for apoptotic markers of DNA cleavage and typical chromatin condensation
by Terminal Deoxynucleotidyl Transferase–mediated dUTP Nick End Labeling (TUNEL) and 4,69-diaminidino-2-phenylindole
(DAPI) staining and electron microscopy. The changes of DYmt (mitochondrial transmembrane potential) and ROS (reactive
oxygen species) indicated that the mitochondria took part in the apoptotic process. Changes in this process detected by
metabonomics and proteomics revealed that the yeast cells tenaciously resisted adversity. Proteins related to redox, cellular
structure, membrane, energy and DNA repair were significantly increased. In this study, the relative changes in the levels of
proteins and metabolites showed the tenacious resistance of yeast cells. However, GSE induced apoptosis in the yeast cells
by destruction of the mitochondrial 60 S ribosomal protein, L14-A, and prevented the conversion of pantothenic acid to
coenzyme A (CoA). The relationship between the proteins and metabolites was analyzed by orthogonal projection
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