a mutation in mtap2 is associated with arrest of mammalian spermatocytes before the first meiotic divisionmtap2的突变与逮捕哺乳动物精母细胞第一次减数分裂前的水平.pdfVIP

Genes 2011, 2, 21-35; doi:10.3390/genes2010021 OPEN ACCESS genes ISSN 2073-4425 /journal/genes Article A Mutation in Mtap2 Is Associated with Arrest of Mammalian Spermatocytes before the First Meiotic Division Fengyun Sun and Mary Ann Handel * The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA; E-Mail: fengyun.sun@ * Author to whom correspondence should be addressed; E-Mail: maryann.handel@; Tel.: +1-207-288-6778; Fax: +1-207-288-6073. Received: 25 October 2010; in revised form: 7 December 2010 / Accepted: 15 December 2010 / Published: 10 January 2011 Abstract: In spite of evolutionary conservation of meiosis, many of the genes that control mammalian meiosis are still unknown. We report here that the ENU-induced repro4 mutation, identified in a screen to uncover genes that control mouse meiosis, causes failure of spermatocytes to exit meiotic prophase I via the G2/MI transition. Major events of meiotic prophase I occurred normally in affected spermatocytes and known regulators of the meiotic G2/MI transition were present and functional. Deep sequencing of mutant DNA revealed a mutation located in an intron of the Mtap2 gene, encoding microtubule-associated protein 2, and levels of Mtap2 transcript were reduced in mutant testes. This evidence implicates MTAP2 as required directly or indirectly for completion of meiosis and normal spermatogenesis in mammals. Keywords: meiosis; spermatogenesis; microtubule-assoc