amyloid precursor protein binding protein-1 modulates cell cycle progression in fetal neural stem cells淀粉样前体蛋白结合蛋白1在胎儿神经干细胞调节细胞周期进程.pdfVIP

Amyloid Precursor Protein Binding Protein-1 Modulates Cell Cycle Progression in Fetal Neural Stem Cells 1 1 1 1 1 1 Yuyoung Joo , Sungji Ha , Bo-Hyun Hong , Jeong a Kim , Keun-A Chang , Hyunjeong Liew , Seonghan 1 2 3 4 1 1,5 Kim , Woong Sun , Joung-Hun Kim , Young Hae Chong , Yoo-Hun Suh , Hye-Sun Kim * 1 Department of Pharmacology, College of Medicine, Seoul National University, Seoul, Republic of Korea, 2 Department of Anatomy, School of Medicine, Korea University, Seoul, Republic of Korea, 3 Department of Life Science, Pohang University of Science and Technology, Pohang, Republic of Korea, 4 Department of Microbiology, School of Medicine, Ewha Womans University, Seoul, Republic of Korea, 5 Seoul National University Bundang Hospital, Seoul National University College of Medicine, Sungnam, Republic of Korea Abstract Amyloid precursor protein binding protein-1 (APP-BP1) binds to the carboxyl terminus of the amyloid precursor protein (APP) and serves as the bipartite activation enzyme for the ubiquitin-like protein, NEDD8. In the present study, we explored the physiological role of APP-BP1 in the cell cycle progression of fetal neural stem cells. Our results show that cell cycle progression of the cells is arrested at the G1 phase by depletion of APP-BP1, which results in a marked decrease in the proliferation of the cells. This action of APP-BP1 is antagonistically regulated by the interaction with APP. Consistent with the evidence that APP-BP1 function is critical for cell cycle progression, the amount of APP-BP1 varies depending upon cell cycle phase, with culminating expression