antioxidant activity of mulberry fruit extracts桑椹提取物的抗氧化活性.pdfVIP

Int. J. Mol. Sci. 2012, 13, 2472-2480; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Antioxidant Activity of Mulberry Fruit Extracts Muhammad Arfan 1, Rasool Khan 1, Anna Rybarczyk 2 and Ryszard Amarowicz 2,* 1 Department of Chemistry, Palosa Campus, Abdul Wali Khan University, 24420 Charsadda, KPK, Pakistan; E-Mails: m_arfan@.pk (M.A.); rasoolkhan1@ (R.K.) 2 Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima Street 10, 10-747 Olsztyn, Poland; E-Mail: a.rybarczyk@pan.olsztyn.pl * Author to whom correspondence should be addressed; E-Mail: r.amarowicz@pan.olsztyn.pl; Tel.: +48-895-234-627; Fax: +48-895-240-124. Received: 13 December 2011; in revised form: 16 February 2012 / Accepted: 16 February 2012 / Published: 22 February 2012 Abstract: Phenolic compounds were extracted from the fruits of Morus nigra and Morus alba using methanol and acetone. The sugar-free extracts (SFEs) were prepared using Amberlite XAD-16 column chromatography. All of the SFEs exhibited antioxidant potential as determined by ABTS (0.75–1.25 mmol Trolox/g), DPPH (2,2-diphenyl-1-picrylhydrazyl) (EC50 from 48 μg/mL to 79 μg/mL), and reducing power assays. However, a stronger activity was noted for the SFEs obtained from Morus nigra fruits. These extracts also