applications of next-generation sequencing technologies to diagnostic virology应用新一代测序技术的诊断病毒学.pdfVIP

Int. J. Mol. Sci. 2011, 12, 7861-7884; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Review Applications of Next-Generation Sequencing Technologies to Diagnostic Virology Luisa Barzon 1,*, Enrico Lavezzo 1, Valentina Militello 1, Stefano Toppo 2 and Giorgio Palù 1 1 Department of Histology, Microbiology, and Medical Biotechnologies, University of Padova, I-35121 Padova, Italy; E-Mails: enrico.lavezzo@unipd.it (E.L.); itello@unipd.it (V.M.); giorgio.palu@unipd.it (G.P.) 2 Department of Biological Chemistry, University of Padova, I-35121 Padova, Italy; E-Mail: stefano.toppo@unipd.it * Author to whom correspondence should be addressed; E-Mail: luisa.barzon@unipd.it; Tel.: +39-049-8218946; Fax: +39-049-8272355. Received: 2 September 2011; in revised form: 20 October 2011 / Accepted: 7 November 2011 / Published: 14 November 2011 Abstract: Novel DNA sequencing techniques, referred to as “next-generation” sequencing (NGS), provide high speed and throughput that can produce an enormous volume of sequences with many possible applications in research and diagnostic settings. In this article, we provide an overview of the many applications of NGS in diagnostic virology. NGS techniques have been used for high-throughput whole viral genome sequencing, such as sequencing of new influenza viruses, for detection of viral genome